Phage Toggle

Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display--A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike.

Lambda phage - Enterobacteria phage λ (lambda phage) is a temperate phage that lives in E. coli.

Enterobacteria phage T4 - Enterobacteria phage T4 is a phage that infects E. coli bacteria.

Phi-X174 phage - The Phi-X174 phage was the first organism to have its genome sequenced.

Phage (Voyager episode) - Phage is the fifth episode of the television series Star Trek: Voyager.

phagetoggle

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